The GL261 cell line is a murine glioma model derived from C57BL/6 mice. This cell line is widely used in neuro-oncology research due to its ability to closely mimic the aggressive and invasive characteristics of human glioblastoma multiforme (GBM). GL261 cells grow as adherent cultures and form tumors when injected intracranially into syngeneic hosts, making them an ideal model for studying glioma progression, tumor microenvironment interactions, and therapeutic responses in an immunocompetent setting.